Soil heat flux plays an important role in energy flux exchange between land and atmosphere, especially in arid and semi-arid region. Based on soil heat flux at Zhangye National Climate Observatory, the diurnal variation of soil heat flux and the relationship between that and radiation flux are analyzed under different weather conditions for clear, cloudy and rainy day.The results show that the soil accepted heat from the air on clear day, on the contrary, the output heat of soil exceeded the input on cloudy and rainy day. In addition, most values of soil heat flux are negative in autumn and winter season, and positive in spring and summer .